(Polyoxyethyleneglycol dodecyl ether) is a commonly used detergent in HPLC applications. Among its uses is its inclusion in the preparation of o-pthalaldehyde and ninhydrin solutions for derivatization of amino acids. Brij-35 has also been used to prevent nonspecific binding to gel filtration and affinity chromatography supports. Brij is a non-ionic detergent useful for the isolation of functional membrane complexes. Brij detergent is difficult to remove from solution by dialysis; however, it can be removed from solution by Extracti-Gel D detergent removing gel. The detergent is compatible with the BCA Protein Assay and has characteristically low UV absorbance at 280 nm.

Also known as: Lauryl Alcohol Ethylene Oxide, Ethoxylated Lauryl Alcohol

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