Cloning
Categories | Subtractive Cloning
Subtractive cloning is a powerful technique that allows isolation of the differences in the nucleic acid composition of two cell samples. Differences can be at the level of RNA species represented within each sample or within the complement of genomic DNAs. Such differences include genes whose differential expression distinguishes one cell type from another, one growth phase from another, or a ormal state from a diseased state.
http://web.wi.mit.edu/sive/pub/Lab%20Resources%20Documents/Sagerstrom%20SubCloning.pdf
Full Site Details Added: 29.11.2007
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| S1 Nuclease to Produce Blunt DNA Ends (Bart Frank)
S1 nuclease removes 5' and 3' overhanging single-stranded DNA and hairpin loops. These may occur after restriction enzyme digestions and in cDNA production. Be careful with S1 nuclease. The acidic conditions can depurinate your DNA, and the enzyme may nick double-stranded DNA.
http://omrf.ouhsc.edu/~frank/S1.html
Full Site Details Added: 22.11.2007
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| Fill-in Reactions to Produce Blunt DNA Ends (Bart Frank)
"Fill-in" reactions are used to convert the 3'-OH ends on DNA molecule that have 5' overhanging ends to blunt ends. Fill-in reactions are inefficient for DNA with 3'overhanging ends.
http://omrf.ouhsc.edu/~frank/FILL-IN.html
Full Site Details Added: 13.12.2007
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