Cosmid
| Plasmid or Cosmid DNA Miniprep
This protocol can be used to isolate sufficient amount DNA from 1.5ml o/n culture or 3ml 6hr culture to do several enzyme digestions.
http://www.hybtech.org/Liu/pcminiprep.html
Full Site Details Added: 04.08.2007
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| Transformation of Plasmids/Cosmids into E. coli (Helen Donis-Keller Lab)
To utilize competent E. coli bacteria to replicate a specific DNA fragment. Three methods are presented transformation of intact plasmids/cosmids ,transformation of ligation mixes and color selectability procedures.
http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid08.html
Full Site Details Added: 14.11.2007
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| Alkaline Lysis Minipreps of Plasmid or Cosmid DNA (Helen Donis-Keller Lab)
Plasmid or cosmid containing E. coli cells are grown and lysed with alkali. The cell debris and chromosomal DNA is precipitated with SDS and potassium acetate. After pelleting the debris the plasmid/cosmid DNA is precipitated from the supernatant with iso
http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid05.html
Full Site Details Added: 13.11.2007
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| Preparation of Miniprep Cosmid DNA from 50 ml Cultures (Helen Donis-Keller Lab)
Cosmid vectors containing foreign DNA inserts are known to replicate more slowly than small plasmids in culture. One of the reasons is believed to be the capacity of the cosmids to take larger insert DNAs (up to 45 kb) causing an increased DNA replication
http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid12.html
Full Site Details Added: 03.08.2007
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