Biology Protocols- Molecular Biology > DNA

PR: 4

DNA Insert Ligation into Vector DNA with T4 DNA Ligase (Fermentas)

http://www.fermentas.com/techinfo/modifyingenzymes/protocols/p_dnainslig.htm
Full Site Details Added: 03.05.2008

PR: 3

DNA Ligations (Bart Frank)

http://omrf.ouhsc.edu/~frank/LIGATION.html
Full Site Details Added: 13.05.2008

PR: 3

DNA Ligation Protocol (Gannon and Powell)
DNA ligation Method, Equipment and reagents, T4 DNA ligase.
http://www.oup.co.uk/pdf/pas/9v1-7-9.pdf
Full Site Details Added: 08.05.2008

PR: 3

Protocol for the quick ligation with T4 DNA ligase (Hinck Lab)
Quick Ligation using PEG 6000 and T4 DNA ligase.
http://hincklab.uthscsa.edu/html/protocols/quick_ligation_protocol.shtml
Full Site Details Added: 30.04.2008

PR: 2

Standard Ligation (Schneitz Lab)

http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/molecularCloning/standardLigation.html
Full Site Details Added: 30.04.2008

PR: 2

Cohesive End Ligation (Chen Lab, Univ. College London)

http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/ligation.html
Full Site Details Added: 15.05.2008

PR: 0

Ligations in Low Melting Temperature Agarose (Helen Donis-Keller Lab)
Ligation of plasmid vector and insert DNAs is carried out directly in melted agarose gel slices. This method is used for subcloning well defined restriction fragments and is probably not suitable for genomic library construction. The method works for both
http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid09.html
Full Site Details Added: 01.05.2008

N/A

DNA ligation with Linearized DNA (Bruce Roe, Univ. Oklahoma)
DNA ligations are performed by incubating DNA insert with linearized cloning vector in the presence of ligation buffer, rATP, and T4 DNA ligase.
http://www.genome.ou.edu/protocol_book/protocol_partII.html#II.D
Full Site Details Added: 06.05.2008

DNA Ligation

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