Biology Protocols- Molecular Biology

PR: 3

Polymerase Chain Reaction (Bart Frank)

http://omrf.ouhsc.edu/~frank/PCR.html
Full Site Details Added: 04.05.2008

PR: 0

Purification of Synthetic Oligonucleotides by Ion Exchange Chromatography (Helen Donis-Keller Lab)
This is a method for purifying synthetic oligonucleotides for use in PCR or sequencing reactions by ion exchange chromatography on a DEAE Sephadex column. While this purification step may be unnecessary for most purposes, it is relatively simple and quick
http://humgen.wustl.edu/hdk_lab_manual/pcr/pcr2.html
Full Site Details Added: 17.04.2008

PR: 0

Polymerase Chain Reaction (Helen Donis-Keller Lab)
The polymerase chain reaction (PCR) is a method for oligonucleotide primer directed enzymatic amplification of a specific DNA sequence of interest. This technique is capable of amplifying a sequence 105 to 106-fold from nanogram amounts of template DNA.
http://humgen.wustl.edu/hdk_lab_manual/pcr/pcr1.html
Full Site Details Added: 24.04.2008

N/A

Purification and Sequencing of PCR Product (Helen Donis-Keller Lab)
This method is used to sequence directly a specific PCR product after large scale amplification. The reaction should be carried out in the usual fashion (see "PCR Method"), except that after optimization of annealing temperature and other conditions in th
http://humgen.wustl.edu/hdk_lab_manual/pcr/pcr3.html
Full Site Details Added: 28.11.2007

PCR

Categories

Colony PCR (0)

Extended PCR (0)

In-situ PCR (0)

PCR Optimization (0)

Quantitative PCR (0)

Real Time PCR (4)

RT-PCR (0)

Standard PCR (0)

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