Biology Protocols- Molecular Biology > PCR

PR: 5

Protocol for Real-Time RT-PCR Using SYBR Green (PGA Project)
describes the detailed experimental procedure for real-time RT-PCR using SYBR Green I as mentioned in Xiaowei Wang and Brian Seed (2003)
http://pga.mgh.harvard.edu/primerbank/PCR_protocol.html
Full Site Details Added: 08.05.2008

PR: 5

Two Step real time PCR protocol (Bryan C. Frank)
Two Step real time PCR protocol.
http://pga.tigr.org/sop/RT-PCR.pdf
Full Site Details Added: 08.05.2008

PR: 5

Real-Time PCR Guide - Informative Background (M.Tevfik Dorak, MD, PhD)
Real-Time PCR Guide - Informative Background.
http://www.dorak.info/genetics/realtime.html
Full Site Details Added: 14.05.2008

PR: 2

Real-Time PCR (qPCR) Basics (Rob Powell)
Learn Quantitative PCR Methodology. Assay parameters, Melt Curves and Primer Dimers, Standard Curves, Dynamic Range, Quantification methods, Delta CT, Standard Curves.
http://web.archive.org/web/20040711051207/http://primerdesign.co.uk/primerdesign/qPCR_basics.html
Full Site Details Added: 16.05.2008

Real Time PCR

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