Phage
| Gel Assay to Determine DNA Content of Phage Lysates (Helen Donis-Keller)
A crude lysate gel assay can be performed to roughly quantitate the DNA in lysates. This is often a valuable time saving step to determine if the phage yield is sufficient to warrant continuing the procedure.
http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda3.html
Full Site Details Added: 01.10.2007
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| Liquid Phage Lysates (Helen Donis-Keller)
5 ml liquid lysates are prepared when a small amount of DNA from a large number of lambda clones is needed. The lysates can be made using 10- 20 ul of a stock lysate or a 100-fold amplified phage "macroplaque" as the inoculum.
http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda2.html
Full Site Details Added: 29.11.2007
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| Assay for Phage Containing the ß- Galactosidase Gene
This assay is used when working with phage vectors carrying the beta-galactosidase gene (often used for immunological screening). If the cloning event disrupts a normally functional copy of the gene in the vector the resulting plaques would appear clear i
http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda7.html
Full Site Details Added: 25.11.2007
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| Long-Term Lambda Phage Storage (Helen Donis-Keller)
No special treatment is required to prepare a lysate for the active collection. The following procedure should be used for long-term storage of lambda clones in the archival collections. The phage are diluted in media containing 7% DMSO and frozen at -80
http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda6.html
Full Site Details Added: 13.08.2007
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| Phage Plate Stock Lysates (Helen Donis-Keller)
To prepare phage lysates to be used for small or large scale phage DNA preps. This method usually produces lysates with titers of 2-8x10e10 pfu/ml.
http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda5.html
Full Site Details Added: 29.11.2007
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| Titering Lambda Phages (Helen Donis-Keller)
This procedure is used to find the number of viable phage particles in a lysate. The phage lysate is serially diluted. A bacterial host strain is inoculated with an appropriate amount of diluted phage and plated out onto growth medium. The plates are coun
http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda4.html
Full Site Details Added: 04.11.2007
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| Streaking Lambda Phages (Helen Donis-Keller)
Phage are streaked onto a medium to obtain an independent isolate prior to preparing a new lysate. This is done to reduce the likelihood of working with lysates which have become contaminated, and/or have accumulated mutations.
http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda12.html
Full Site Details Added: 15.10.2007
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