Yeast Artificial Chromosome
Categories | High Molecular Weight Yeast Liquid DNA Preparation (Helen Donis-Keller)
To isolate intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. One can expect a yield of 100-200 µg of DNA per prep.
http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast4.html
Full Site Details Added: 09.02.2007
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| Yeast DNA Minipreps (Helen Donis-Keller)
To rapidly isolate genomic DNA from yeast cells. Up to 24 YAC clone samples can be processed in one day as opposed to sucrose gradient preps which require 3 days. This method is designed for < 1 g wet weight of cells. Because care is not taken to prevent
http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast3.html
Full Site Details Added: 12.11.2007
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| Long-Term Storage of Yeast Stocks (Helen Donis-Keller)
Yeast strains may be stored indefinitely at low temperatures (-80 degrees C). It is lab policy to prepare a frozen stock of newly acquired or created strains for the archives as soon as possible after beginning to work with it. Two archiving method
http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast2.html
Full Site Details Added: 05.12.2007
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| Screening YAC libraries (Helen Donis-Keller)
This is a method for screening YAC libraries for specific DNA sequences. Transformants are patched onto selective medium plates and colonies lifted to nylon filters for further growth. Cells are then lysed in place and the DNA is fixed to the filters. DN
http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast7.html
Full Site Details Added: 09.02.2007
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