S1 Nuclease to Produce Blunt DNA Ends - Details
Link Details
| ID: | 18373 | | Title: | S1 Nuclease to Produce Blunt DNA Ends - http://omrf.ouhsc.edu/~frank/S1.html | | Pagerank: | 3 | | Description: | S1 nuclease removes 5' and 3' overhanging single-stranded DNA and hairpin loops. These may occur after restriction enzyme digestions and in cDNA production. Be careful with S1 nuclease. The acidic conditions can depurinate your DNA, and the enzyme may nick double-stranded DNA. | | Category: | Molecular Biology: Cloning | | Contributed by: | Bart Frank | | Link Owner: | | | Date Added: | February 18, 2007 09:06:57 PM | | Number Hits: | 6 | | |
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